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BIO-CHO-1 SFM Base is a serum-free medium that is the fundamental or base formulation designed and modified for Chinese Hamster Ovary (CHO) cells in monolayer culture to optimize cell growth and protein expression. This medium is utilized for the growth of an array of CHO cells such as the CHO-K1 as well as transfected cells containing recombinant DNA which is related to the DHFR gene. BIO-CHO-1 is used in conjunction with the BIO-GRO-CHO SFM Supplement. Supplement is 100 fold concentrate which contains proteins and other components that require storage at -20°C.