Colcemid (Demecolcine) Solution
Colcemid for chromosome analysis during lymphocyte karyotyping and amniotic fluid cell chromosome analysis, and in cell synchronization.
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Colcemid, N-deacetyl-N-Methylcolchicine, is related to colchicine, but animal studies found it to be much less toxic. Colcemid arrests mitotic cultured cells in metaphase and it should be treated carefully, since it is mutagenic, tumorigenic, and teratogenic. Colcemid Solution from Biological Industries is prepared in phosphate buffered saline (PBS) and it is recommended to use a concentration of 0.1 µg/ml in culture medium. Colcemid is recommended for use in chromosome analysis during lymphocyte karyotyping and amniotic fluid cell chromosome analysis, and in cell synchronization. Store the Colcemid Solution at 2-8ºC, protected from light.