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EZ-Block is used in hybridization and detection procedures using non-radioactive nucleic acids probes, and for Western blots. When immunoassays and hybridization assays, such as dot blots, Western blots, Southern blots, or Northern blots are performed, there is nonspecific binding resulting in high background. In order to reduce the nonspecific binding, EZ-Block reagent is used to “block” unbound sites left after immobilization of the specific protein or after the hybridization with non-radioactive probe. EZ-Block improves sensitivity and reduces background.
Note: Nonfat dry milk inhibits the streptavidin-biotin interaction due to its content of biotin