Serum-Free Cell Freezing Medium

Description

Serum-Free Cell Freezing Medium

The Serum-Free Cell Freezing Medium is a ready-to-use, animal component-free, serum-free, and protein-free cryopreservation solution optimised for various cell lines.  When culturing cells in a serum-free environment, it is vital to also maintain serum-free conditions during cryopreservation.

The Serum-Free Cell Freezing Medium has been shown to result in high rates of cell viability, proliferation, adherence (in appropriate lines), and bioactivity/ expression following freezing and thawing.  Superior results were observed when comparing both serum-containing freezing media as well as competing serum-free products. This freezing medium is therefore an ideal product for both serum-containing and serum-free applications.

The Serum-Free Cell Freezing Medium has been extensively tested on multiple cell lines, including 3T3, BGM, vero cells, MRC-5, HEK-293, HEp-2, BSC-1, BF16-F10, BHK-21, CHO, HELA, MA-10, and mesenchymal cells.

Advantages

• Animal component-free, serum-free, protein-free solution
• CE mark
• Ready-to-use solution, simple protocol
• Cryoprotective formulation designed to minimize dehydration effects
• Effective maintenance of cell viability, adhesion, and bioactivity
• Suitable for use in cell banks and biopharmaceutical applications

Instructions For Use

Cryopreservation of serum-free cell cultures 

1. For freezing adherent cells, detach cells using the appropriate dissociation solution. (For freezing of cells in suspention, skip to step 2.)
2. Centrifuge to pellet the cells at 200 – 300 x g for 3 to 5 minutes.
3. Suspend the cell pellet in cold Serum-Free Cell Freezing Medium at a concentration of 3 to 5 million cells/mL.
4. Freeze the cells gradually (1 to 2°C per minute) and store them in liquid nitrogen.
5. Viability and recovery of cryopreserved cells should be evaluated 24 hours after storage of vials in liquid nitrogen by following the thawing procedure outlined below.

Thawing cryopreserved cells 

1. Thawing should be performed at 37°C.
2. Immediately after thawing, suspend the cells in serum-free growth medium at a ratio of at least 1:10.
3. Centrifuge and suspend in growth medium as desired.
4. Culture the cells according to the recommended seeding density.

Additional information

1. E. Nigro, E. Soprana, A. Brini, et l., An Antitumor Cellular Vaccine Based on a Mini-Membrane IgE. J Immunol 188:103-110 (2012).
2. G. Di Lullo, E. Soprana, M. Panigada, et al. The combination of marker gene swapping and fluorescence-activated cell sorting improves the efficiency of recombinant modified vaccinia virus Ankara vaccine production for human use. J. of Virological Methods, 163, Issue 2: 195–204 (2010)
3. Amit, Michal, and Joseph Itskovitz-Eldor. Novel methods and culture media for culturing pluripotent stem cells. U.S. Patent Application 13/821,244.
4. De Falco, Elena, et al. A standardized laboratory and surgical method for in vitro culture isolation and expansion of primary human Tenon’s fibroblasts.Cell and tissue banking 14.2 (2013): 277-287.

Materials Safety Data Sheet

• Serum-Free Cell Freezing Medium (MSDS)

Manuals and Protocols

• Serun-Free Cell Freezing Medium Instructions for Use