Cryostem hPSC Freezing Medium

Cryostem hPSC Freezing Medium

Chemically defined, animal component-free, protein-free cryopreservation solution for human ES cells and iPS cells.
CryoStem™ Freezing Medium05-710-1D10mL
CryoStem™ Freezing Medium05-710-1E50 mL
  • Description
  • Specifications
  • References
  • Documentation
  • Reviews (0)


Cryostem hPSC Overview

CryoStem™ hPSC Freezing Medium is a ready-to-use solution for the cryopreservation of human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells in serum-free and animal component-free conditions. Pluipotent stem cells preserved with CryoStem™ hPSC Freezing Medium demonstrate high viability and recovery after thawing, and express typical pluripotent markers in culture. CryoStem Freezing Medium maintains xeno-free conditions during cryopreservation when your cells have been cultured in a xeno-free system.

CryoStem hPSC Freezing Medium has been validated on human ES cells (H1, H9 and HuES9). Superior results were seen when used in comparison with both serum-containing freezing media as well as competing serum-free freezing solutions.

CryoStem is a novel solution composing of methylcellulose and DMSO therefore eliminating the risks associated with sera and serum-derived products.


  • Chemically defined, Animal component-free (ACF), Protein-free
  • Works with various media
  • Suitable for freezing hESC and hiPSC cultured in both feeder and feeder-free conditions
  • High recovery efficiency: maintains superior attachment ability as well as growth profile
  • Maintains hESC and hiPSC pluripotency
  • Complete formulation; Ready-to-use at 2-8°C

Additional information




Storage Conditions

2 to 8°C

Instructions for Use

Do not use if a visible precipitate is observed in the medium. Do not use beyond the expiration date indicated on the product label.


CryoStem Freezing Medium is intended for in vitro diagnostic use


10mL, 50 mL

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  2. S. Reichman et al. Generation of Storable Retinal Organoids and Retinal Pigmented Epithelium from Adherent Human iPS Cells in Xeno‐Free and Feeder‐Free Conditions.STEM CELLS 35.5 (2017): 1176-1188.
  3. F. Pistollato, et al. Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing. JoVE (Journal of Visualized Experiments) 124 (2017): e55702-e55702.
  4. L. Tian et al. Efficient and controlled generation of 2D and 3D bile duct tissue from human pluripotent stem cell-derived spheroids." Stem Cell Reviews and Reports 12.4 (2016): 500-508.
  5. J. Chal et al. Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro.Nature protocols 11.10 (2016): 1833-1850.
  6. M. S. Y Hiew. Generation and Microrna Expression Profiling of Colorectal Cancer Cell-Derived Induced Pluripotent Cancer Cells. Diss. UTAR, 2016.
  7. N. Nishishita et al. An effective freezing/thawing method for human pluripotent stem cells cultured in chemically-defined and feeder-free conditions. American journal of stem cells 4.1 (2015): 38.
  8. Z. Danhong, et al. Method of cryopreservation of stem cell-derived retinal pigment epithelial cells on polymeric substrate.U.S. Patent Application No. 14/114,182, 2014
  9. C. Martin et al. Highly Efficient microRNA-Enhanced mRNA Reprogramming of Diseased Human Fibroblasts in a Feeder-Free Culture System. STEMGENT Application Note, 2014
  10. C. Buensuceso et al. Induced pluripotent stem cells prepared from human kidney-derived cells. US Patent 20140073049 A1, 2014
  11. R. Sivapatham, X.Zeng. Generation and Characterization of Patient-Specific Induced Pluripotent Stem Cell for Disease Modeling. Methods in Molecular Biology, 2014
  12. L. Tian, N. Prasad, Y. Jang. In Vitro Modeling of Alcohol-Induced Liver Injury Using Human-Induced Pluripotent Stem Cells. Methods in Molecular Biology, 2014
  13. C. Buensuceso et al. Induced pluripotent stem cells from human umbilical cord tissue-derived cells. US Patent 20130157365, 2013
  14. S. Hikita et al. Methods of Culturing Retinal Pigmented Epithelium Cells, Including Xeno-Free Production, RPE Enrichment, and Cryopreservation. US Patent 20130196369 A1, 2013
  15. N. Nishishita et al. Generation of Virus-Free Induced Pluripotent Stem Cell Clones on a Synthetic Matrix via a Single Cell Subcloning in the Naïve State. PLoS ONE 7(6): e38389. doi:10.1371/journal.pone.0038389 , 2012
  16. F. Pistollato et al. Standardization of pluripotent stem cell cultures for toxicity testing. Vol. 8, No. 2 , Pages 239-257 (doi:10.1517/17425255.2012.639763), 2012


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