CryoStem™ MSC Freezing Medium

CryoStem™ MSC Freezing Medium

Chemically defined, animal component-free, protein-free solution

Designed for optimal cryopreservation of hMSCs

CryoStem™ MSC Freezing Medium, 50 mL05-712-1E50 mL
CryoStem™ MSC Freezing Medium, 10 mL05-712-1D10 mL
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CryoStem MSC Freezing Medium Overview:

CryoStem MSC Freezing Medium is a chemically defined, animal component-free and protein-free formulation, designed for optimal cryopreservation of human mesenchymal stem cells from a variety of sources, including bone marrow (BM-MSC), adipose tissue (AT-MSC) and umbilical cord tissue (UC-MSC).

When culturing cells under serum free conditions, it is important to cryopreserve the cells also in a solution free of serum.  The novel CryoStem™ MSC Freezing Medium contains methylcellulose and DMSO as a replacement for serum.  After freezing and thawing, a very high percentage of viable hMSCs are obtained with excellent attachment ability as well as morphology and growth performance.

Comparative studies have shown that in most cases higher viabilities and adhesion percentages are obtained in comparison to serum-containing freezing medium. This means that CryoStem™ MSC Freezing Medium can be also be used on hMSC that have been cultured in serum supplemented growth media.

Figure: Recovery of hMSC-BM after thawing procedure. Cells were frozen using CryoStem™ MSC Freezing Medium, thawed and re-seeded in MSC NutriStem® XF on pre-coated plates. Representative images were taken at the indicated time points post-thawing (x200).












  • Chemically defined, animal component-free, protein-free
  • Complete, ready-to-use solution
  • Provides optimum cryopreservation conditions for hMSCs from a variety of sources
  • High cell attachment and viability after thawing
  • Suitable for cells cultured in either serum-free or serum-containing media
  • Optimised for hMSCs cultured with MSC NutriStem® XF Medium



Additional information




Storage Conditions


Quality Control

This product is tested for performance using human mesenchymal stem cells (hMSC). Each lot is tested for optimal cell viability, cell attachment, and proliferation after cryopreservation and thawing.


10 mL, 50 mL


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