Serum-Free Cell Freezing Medium
A chemically defined, animal component-free, protein-free cryopreservation solution
|Serum-Free Cell Freezing Medium, 20mL||05-065-1C||20 mL|
|Serum-Free Cell Freezing Medium, 500mL||05-065-1A||500 mL|
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Serum-Free Cell Freezing Medium
The Serum-Free Cell Freezing Medium is a ready-to-use, animal component-free, serum-free, and protein-free cryopreservation solution optimised for various cell lines. When culturing cells in a serum-free environment, it is vital to also maintain serum-free conditions during cryopreservation.
The Serum-Free Cell Freezing Medium has been shown to result in high rates of cell viability, proliferation, adherence (in appropriate lines), and bioactivity/ expression following freezing and thawing. Superior results were observed when comparing both serum-containing freezing media as well as competing serum-free products. This freezing medium is therefore an ideal product for both serum-containing and serum-free applications.
The Serum-Free Cell Freezing Medium has been extensively tested on multiple cell lines, including 3T3, BGM, vero cells, MRC-5, HEK-293, HEp-2, BSC-1, BF16-F10, BHK-21, CHO, HELA, MA-10, and mesenchymal cells.
- Animal component-free, serum-free, protein-free solution
- CE mark
- Ready-to-use solution, simple protocol
- Cryoprotective formulation designed to minimize dehydration effects
- Effective maintenance of cell viability, adhesion, and bioactivity
- Suitable for use in cell banks and biopharmaceutical applications
Instructions For Use
Cryopreservation of serum-free cell cultures
- For freezing adherent cells, detach cells using the appropriate dissociation solution. (For freezing of cells in suspention, skip to step 2.)
- Centrifuge to pellet the cells at 200 – 300 x g for 3 to 5 minutes.
- Suspend the cell pellet in cold Serum-Free Cell Freezing Medium at a concentration of 3 to 5 million cells/mL.
- Freeze the cells gradually (1 to 2°C per minute) and store them in liquid nitrogen.
- Viability and recovery of cryopreserved cells should be evaluated 24 hours after storage of vials in liquid nitrogen by following the thawing procedure outlined below.
Thawing cryopreserved cells
- Thawing should be performed at 37°C.
- Immediately after thawing, suspend the cells in serum-free growth medium at a ratio of at least 1:10.
- Centrifuge and suspend in growth medium as desired.
- Culture the cells according to the recommended seeding density.
20 mL, 500 mL
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- G. Di Lullo, E. Soprana, M. Panigada, et al. The combination of marker gene swapping and fluorescence-activated cell sorting improves the efficiency of recombinant modified vaccinia virus Ankara vaccine production for human use. J. of Virological Methods, 163, Issue 2: 195–204 (2010)
- Amit, Michal, and Joseph Itskovitz-Eldor. Novel methods and culture media for culturing pluripotent stem cells. U.S. Patent Application 13/821,244.
- De Falco, Elena, et al. A standardized laboratory and surgical method for in vitro culture isolation and expansion of primary human Tenon’s fibroblasts.Cell and tissue banking 14.2 (2013): 277-287.
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