BIOTARGET-1 SFM

BIOTARGET-1 SFM

Serum-free, xeno-free medium, optimized specifically for culturing PBMCs, 500 mL
SKU: 05-080-1A
Category:
  • Description
  • Specifications
  • References
  • Documentation
  • Reviews (0)

Description

BIOTARGET-1 SFM Overview:

BIOTARGET-1 SFM is a serum-free, xeno-free medium specially developed and optimised for culturing PBMC – peripheral blood mononuclear cells (lymphocytes and monocytes). This medium is available in several customised packaging configurations to suit your experimental needs, including Sartorius-Stedim cultibags.

Advantages

  • Xeno-free
  • Chemically defined
  • No added cytokines
  • Comprehensive validation studies
  • CE Mark
  • Manufactured in ISO 13485:2003 and ISO 9001:2008 certified facility

Applications

  • Mitogen mediated activation of peripheral blood mononuclear cells (PHA, CON.A, OKT-3)
  • Lymphoid cell mediated activation of mononuclear cells (RAJI, PEER, BA, MOLT-4, JURKAT)
  • IL-2 and IL-3 production from mononuclear cells
  • Long-term post- activation culture of mononuclear cells
  • LAK / TIL cell generation by mononuclear cell interleukin-2 mediated activation
  • Natural killer cell (NK) generation by mononuclear cell activation
  • Cytotoxic T cell generation by mononuclear cell activation
  • Activation of macrophages
  • Investigating various cytokines’ influence on the production of mononuclear cell sub-populations
  • HIV proliferation
  • Retrovirus proliferation in T cells for vaccine development

Additional information

Quantity

500 mL

Glutamine

No Glutamine

Storage Conditions

2-8°C

Shipping Conditions

Cold Pack

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  12. Yang-Ming Tseng, Sheng-Yi Chen, Chien-Hung Chen, et al.,. Effects of Alcohol-Induced Human Peripheral Blood Mononuclear Cell (PBMC) Pretreated Whey Protein Concentrate (WPC) on Oxidative Damage. Journal of Agricultural and Food Chemistry, 56 (17): 8141–8147, 2008.
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  15. C. Rabinowitz and B. Rinkevich. Epithelial cell cultures from Botryllus schlosseri palleal buds: accomplishments and challenges. Methods in Cell Science 25 (3-4), 2004.
  16. F. Martí, E. Bertran, M. Llucià, et al., Platelet factor 4 induces human natural killer cells to synthesize and release interleukin-8. Journal of Leukocyte Biology 72: 590-597, 2002.
  17. A. Bishara, R. Malka, C. Brautbar, et al., Cytokine production in human mixed leukocyte reactions performed in serum-free media. Journal of Immunological Methods 215 (1-2): 187-190, 1998.
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  20. Malka R.; Brautbar C.; Kedar E.; et al., Human mixed leukocyte reaction (MLR) performed in serum-free media and serum-containing medium. Human immunology 44(1) : 137, 1995.

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