EndoGo XF Medium

Description

EndoGo XF Medium Overview:

EndoGo XF Medium is a novel xeno-free culture medium specially designed for long-term expansion of large and small vessels endothelial cells from numerous sources. The medium provides optimally balanced nutritional environment that selectively promotes proliferation of normal human endothelial cells, while maintaining the classic cobblestone-like cell morphology, phenotypic surface marker profile, and angiogenic differentiation potential.
EndoGo™ XF supports human macro and microvascular endothelial cells (MVEC).

Advantages

• Defined, xeno-free, serum-free medium
• Supports long-term expansion of large and small vessels endothelial cells
• Maintains high proliferation potential, typical morphology and EC marker expression

HDMEC (Human Dermal Microvascular Endothelial Cells) proliferation

HPMEC (Human Pulmonary Microvascular Endothelial Cells) proliferation

Cell counts and population doubling level (PDL) of HDMEC and HPMEC expanded for several passages in EndoGo™ XF in comparison to commercial FBS-containing medium. Viable cells were counted using ChemoMetec Viability and Cell Count Assay.

Macro vascular HUVEC (Human Umbilical Vein Endothelial Cells)

A

B

C

Micro vascular HDMEC (Human Dermal Microvascular Endothelial Cells)

Microvascular endothalial cells and macrovascular endothelial cells maintain classic cobblestone like morphology after expansion for several sequential passages with equal seeding (5000 cells/cm2) in EndoGo™ XF +2% OTC human AB serum on hFN pre-coated dishes (A), expanded cells preserved endothelial cell features (EC markers expression) (B) and angiogenic potential to form capillary-like tubes (C).

Instructions For Use:

The complete endothelial cell culture media consist of 2 components: EndoGo™ XF Medium (05-400-1) and EndoGo™ XF Supplement Mix (05-410-1). These individual components can not be perchased separately. The medium does also require the addition of human AB serum (off the clot) or human platelet lysate (not supplied with the media).
EndoGo™ XF Medium is recommended for use in conjunction with Recombinant Trypsin Solution with EDTA (Cat. No. 03-079-1) and Soybean Trypsin Inhibitor (SBTI) (Cat. No. 03-048-1).

Storage and Stability

• EndoGo™ XF Medium should be stored at -20°C.
• EndoGo™ XF Supplement Mix should be stored at -20°C. Avoid repeated freeze/thaw cycles (up to two freeze – thaw cycles max.)
• The complete EndoGo™ XF Medium is stable at 2 to 8°C for up to 30 days.
• Avoid exposure to light

Additional information

Materials Safety Data Sheet

• EndoGo XF Medium (MSDS)
• EndoGo XF Supplement Mix (MSDS)

Manuals and Protocols

• EndoGo™ XF Medium Instructions for Use
• POSTER A Xeno-Free Culture System for Human Microvascular Endothelial Cells from Various Sources –Toward Clinical Applications

Product Literature

• EndoGo™ XF Medium Product Sheet