Nutristem V9 XF Medium

Nutristem V9 XF Medium

Defined, xeno-free, serum-free culture medium for hPSCs optimized for vitronectin

NutriStem® V9 XF Medium, 500mL05-105-1A-US500 mL
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NutriStem V9 Overview

Nutristem V9 XF Medium is a defined, xeno-free, serum-free medium designed to support the growth and expansion of human pluripotent stem cells (hPSC), using vitronectin and enzyme-free passage using EDTA. It contains only the essential components required for long-term maintenance of hES and hiPS cells. NutriStem V9 XF Medium shows superior performance and proliferation rates, whilst retaining the pluripotency of the cells, during long-term cultures.

NutriStem V9 XF Medium allows culture of hPSC in vitronectin pre-coated culture dishes. It can also be used in a pre-coating free protocol, where BI’s ACF Vitronectin can be directly added to the complete V9 medium.

NutriStem V9 Features

  • Produced under cGMP guidelines
  • Maintains human ES and iPS cell pluripotency at high passage
  • Superior results using Vitronectin, may be used with Matrigel coated plates
  • Supports long-term expansion
  • Cytokine-free basal medium, applicable for EB formation, reprogramming, and differentiation
  • Does not contain antibiotics.

Sample Data

Long-term Expansion






Nucleocounts performed on equal volume of cell suspension Chemometech, (count of aggregated cells) during long-term expansion of H1 cultured in NutriStem® V9 XF medium and E8 under a weekend-free feeding regime using 0.5μg/cm2 Vitronectin ACF. NutriStem® V9 XF medium shows superior proliferation rate in long-term culture (over 20 passages)

Cell morphology










Phase contrast images (x100) of H1 hESC culture maintained in NutriStem® V9 XF medium under a weekendfree feeding regime, using 0.5μg/cm2 Vitronectin ACF. Representative images from culture at P6, P16 and P20. Undifferentiated colonies maintained during long-term culture of H1 hESC

Immunofluorescence staining

Tra 1-60










Immunofluorescence analysis of human pluripotent markers of H1 hPSC expanded in NutriStem® V9 XF medium under a weekend-free feeding regime using Vitronectin ACF. Cells from P8 were fixated and stained for the classic pluripotent surface markers: TRA 1-60 (Alexa Fluor) (green) and nuclear conjugated markers: Nanog-RRX, counterstained with DAPI (blue). Scale bar 200μm. Cells cultured in NutriStem® V9 XF Medium on vitronectin express high levels of pluripotent markers


Flow cytometric quantification






Immunophenotyping analysis for human pluripotent markers of H1 hESC culture maintained in NutriStem® V9 XF medium under a weekend-free feeding regime using 0.5μg/cm2 Vitronectin ACF at P8 and P17. Data presented as % expression from gated viable cells. High expression of pluripotent stem cell markers in cells cultured in NutriStem® V9 XF medium on vitronectin

Quality Control

Each lot is tested for performance using H1 hESC by expansion of the cells for 3 passages, IF staining for pluripotency marker, and morphology. Other tests include sterility, pH, osmolality and endotoxins levels.


NutriStem ® V9 XF Basal Medium, 500 mL
NutriStem ® V9 XF Supplement Mix, 1 mL

Cell Type:
Stem Cells (Embryonic), Stem Cells (Human), Stem Cells (iPS – Induced Pluripotent Stem)

Additional information


500 mL




Storage Conditions

Store at -10° to -20°C


NutriStem® is a registered trademark of Biological Industries


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