BIOAMF-2 Medium

Optimized for the primary culture of human amniotic fluid cells and chorionic villi (CV) samples used in prenatal diagnostic testing
BIO-AMF™-2 Medium, 100 mL01-194-1A100 mL
BIO-AMF™-2 Medium, 500 mL01-194-1B500 mL
  • Description
  • Specifications
  • References
  • Documentation
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BIOAMF-2 Overview:

BIOAMF-2 Complete Medium is a fully-supplemented medium specifically designed for the primary culture of human amniotic fluid and chorionic villi cells for cytogenetic studies.


  • Ready-to-use The media comes as a single bottle formulation, complete with L-Glutamine and Gentamycin, which is shipped frozen. Upon receipt, simply thaw and use. We recommend storing thawed medium at 2-8°C for up to seven days.
  • Quality and performance testing BIOAMF-2 Complete Medium is evaluated for cell growth using primary human amniotic fluid cells from a leading clinical cytogenetics laboratory. Additionally we test each batch for sterility, pH, osmolality and endotoxin concentrations.
  • Optimized and complete solution BIOAMF™-2 Complete Medium is prepared with Fetal Bovine Serum (FBS), L-Glutamine and Gentamycin optimized for both open (CO2) and closed systems. cGMP Manufacturing and Quality System BIOAMF™-2 Complete Medium is manufactured at our cGMP compliant facility, located in Beit Haemek, Israel. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Shelf Life

24 months from date of production and stored frozen
After thawing, 7 days at 2°C to 8°C

Additional information


100 mL, 500 mL




Cell Type






Shipping Conditions

Dry Ice

Storage Conditions


  1. Constantinou M, Kałuzewski B, Helszer Z, et al., Prenatal detection of maternal UPD15 in a new case with i(15p) by Timing Replication Test (TRT) and methylation analysis. J. Appl. Genet., 44(2): 209-218 (2003).
  2. Sarig R, Fuchs O, Tencer L, Panski A, Nudel U, et al. Cloned Myogenic Cells Can Transdifferentiate In Vivo into Neuron-Like Cells. PLoS ONE 5(1) (2010).
  3. M. Krkavcová, N. Jančárková, M. Janashia, et al., Genetic alterations in gynecological malignancies. NEOPLASMA 55 (3) (2008).
  4. E. Guetta, G. Barkai, L. Gutstein-Abo.  Trophoblasts Isolated from the Maternal Circulation : In VitroExpansion and Potential Application in Non-invasive Prenatal Diagnosis. Journal of Histochemistry and Cytochemistry 53 (3): 337-339 (2005).
  5. J.M. Bellón, A. Bajo, N. Ga-Honduvilla, et al., . Fibroblasts From the Transversalis Fascia of Young Patients With Direct Inguinal Hernias Show Constitutive MMP-2 Overexpression. Annals of Surgery. 233(2): 287–291 (2001).
  6. U. Ozgen , M. Ikbal, A. Hacimuftuoglu, et al., Fibroblast growth stimulation by extracts and compounds of Onosma argentatum roots. Journal of Ethnopharmacology 104 (1-2, 8): 100-103 (2006).
  1. R. Sarig, Z. Baruchi, O. Fuchs, et al., Regeneration and Transdifferentiation Potential of Muscle-Derived Stem Cells Propagated as Myospheres. Stem Cells 24 (7): 1769-1778 (2006).
  2. Ji Hyeon Park, Jung Hoon Woo, Sung Han Shim, et al., Application of a target array Comparative Genomic Hybridization to prenatal diagnosis. BMC Medical Genetics 11:102 (2010).
  3. S. Han Shim, T. Ki Yoon, D. Hyun Cha, et al., Endothelial Nitric Oxide Synthase (eNOS) gene polymorphisms in spontaneously aborted embryos. Genes & Genomics 32 (3) (2010).
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  5. Y. Gruenbaum-Cohena, I. Harelb, K.B. Umanskya, et al., The actin regulator N-WASp is required for muscle-cell fusion in mice. PNAS July 10 2012 Vol 109 n 28 11211-11216.
  6. C. Mozzetta, S. Consalvi, V. Saccone, et al., Fibroadipogenic progenitors mediate the ability of HDAC inhibitors to promote regeneration in dystrophic muscles of young, but not old Mdx mice. EMBO Molecular Medicine Apr 2013; 5(4): 626–639

Materials Safety Data Sheet

Manuals and Protocols


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