Cryostem hPSC Freezing Medium

Description

Cryostem hPSC Overview

CryoStem™ hPSC Freezing Medium is a ready-to-use solution for the cryopreservation of human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells in serum-free and animal component-free conditions. Pluipotent stem cells preserved with CryoStem™ hPSC Freezing Medium demonstrate high viability and recovery after thawing, and express typical pluripotent markers in culture. CryoStem Freezing Medium maintains xeno-free conditions during cryopreservation when your cells have been cultured in a xeno-free system.

CryoStem hPSC Freezing Medium has been validated on human ES cells (H1, H9 and HuES9). Superior results were seen when used in comparison with both serum-containing freezing media as well as competing serum-free freezing solutions.

CryoStem is a novel solution composing of methylcellulose and DMSO therefore eliminating the risks associated with sera and serum-derived products.

Features

• Chemically defined, Animal component-free (ACF), Protein-free
• Works with various media
• Suitable for freezing hESC and hiPSC cultured in both feeder and feeder-free conditions
• High recovery efficiency: maintains superior attachment ability as well as growth profile
• Maintains hESC and hiPSC pluripotency
• Complete formulation; Ready-to-use at 2-8°C

Additional information

1. G. Guo, et al. Epigenetic resetting of human pluripotency." bioRxiv (2017): 146712.
2. S. Reichman et al. Generation of Storable Retinal Organoids and Retinal Pigmented Epithelium from Adherent Human iPS Cells in Xeno‐Free and Feeder‐Free Conditions.STEM CELLS 35.5 (2017): 1176-1188.
3. F. Pistollato, et al. Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing. JoVE (Journal of Visualized Experiments) 124 (2017): e55702-e55702.
4. L. Tian et al. Efficient and controlled generation of 2D and 3D bile duct tissue from human pluripotent stem cell-derived spheroids." Stem Cell Reviews and Reports 12.4 (2016): 500-508.
5. J. Chal et al. Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro.Nature protocols 11.10 (2016): 1833-1850.
6. M. S. Y Hiew. Generation and Microrna Expression Profiling of Colorectal Cancer Cell-Derived Induced Pluripotent Cancer Cells. Diss. UTAR, 2016.
7. N. Nishishita et al. An effective freezing/thawing method for human pluripotent stem cells cultured in chemically-defined and feeder-free conditions. American journal of stem cells 4.1 (2015): 38.
8. Z. Danhong, et al. Method of cryopreservation of stem cell-derived retinal pigment epithelial cells on polymeric substrate.U.S. Patent Application No. 14/114,182, 2014
9. C. Martin et al. Highly Efficient microRNA-Enhanced mRNA Reprogramming of Diseased Human Fibroblasts in a Feeder-Free Culture System. STEMGENT Application Note, 2014
10. C. Buensuceso et al. Induced pluripotent stem cells prepared from human kidney-derived cells. US Patent 20140073049 A1, 2014
11. R. Sivapatham, X.Zeng. Generation and Characterization of Patient-Specific Induced Pluripotent Stem Cell for Disease Modeling. Methods in Molecular Biology, 2014
12. L. Tian, N. Prasad, Y. Jang. In Vitro Modeling of Alcohol-Induced Liver Injury Using Human-Induced Pluripotent Stem Cells. Methods in Molecular Biology, 2014
13. C. Buensuceso et al. Induced pluripotent stem cells from human umbilical cord tissue-derived cells. US Patent 20130157365, 2013
14. S. Hikita et al. Methods of Culturing Retinal Pigmented Epithelium Cells, Including Xeno-Free Production, RPE Enrichment, and Cryopreservation. US Patent 20130196369 A1, 2013
15. N. Nishishita et al. Generation of Virus-Free Induced Pluripotent Stem Cell Clones on a Synthetic Matrix via a Single Cell Subcloning in the Naïve State. PLoS ONE 7(6): e38389. doi:10.1371/journal.pone.0038389 , 2012
16. F. Pistollato et al. Standardization of pluripotent stem cell cultures for toxicity testing. Vol. 8, No. 2 , Pages 239-257 (doi:10.1517/17425255.2012.639763), 2012

Materials Safety Data Sheet

CryoStem™ Freezing Medium (MSDS)

Manuals and Protocols

CryoStem™ Freezing Medium Instructions for Use

Product Literature

CryoStem™ Freezing Medium Product Sheet

Technical Reports

WiCell – BI-USA CryoStem™ Freezing Medium Lot Qualification Report