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Soybean Trypsin Inhibitor
In a serum-free culture environment, the cells need to be separated by rapid centrifugation or by using trypsin inhibitors such as Soybean Trypsin Inhibitor (SBTI).
SBTI is a single polypeptide that forms a stable, stoichiometric, enzymically inactive complex with trypsin, thereby reducing the availability of trypsin by somewhat binding chymotrypsin.
With Biological Industries’SBTI, any excess Crystalline Trypsin Solution may be completely neutralised, which means that the use of serum can be avoided for the process. The cells can then be successfully re-suspended in an appropriate growth medium.
The complete formulation is available.
Before use, the solution is diluted by a factor of 50 with sterile phosphate buffered saline (PBS) solution, and can then be stored at 4ºC for up to 60 days.